electron microscopy fixation solution  (Servicebio Inc)

Journal: PLOS One

Article Title: Cuproptosis-related genes associated with mitochondrial dysfunction in Parkinson’s disease

doi: 10.1371/journal.pone.0327550

Figure Lengend Snippet: (A) Immunohistochemical analysis of TH in MPTP-induced PD mice model in vivo. Serious loss of TH in SNpc. (B) Immunohistochemical analysis of TH positive area proportion statistics. (C) Observation of mitochondrial structure by transmission electron microscopy. (D) Detection of mitochondrial membrane potential in the SN of MPTP induced PD mice model using JC-1 mitochondrial membrane potential fluorescent probe staining. (E) JC-1 mean fluorescence intensity statistics. (F) JC-1 mitochondrial membrane potential fluorescent probe staining for MPP + induced SH-SY5Y cells in vitro PD model. (G) JC-1 mean fluorescence intensity statistics. (H) Detection of ROS in Parkinson’s disease model induced by MPP + in SH-SY5Y cells in vitro. (I) ROS relative fluorescence intensity statistics. Compared with the Saline group, ** P < 0.01. n = 3.

Article Snippet: Sectioning and electron microscopy fixation of mouse brain SN tissue were performed as follows: The tissue was fixed with electron microscopy fixation solution (G1102, Servicebio, China) at room temperature for 2 h and then stored at 4°C in the dark to maintain the integrity of its cellular structure.

Techniques: Immunohistochemical staining, In Vivo, Transmission Assay, Electron Microscopy, Membrane, Staining, Fluorescence, In Vitro, Saline